Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sequences. 2. DNA ligase joins DNA fragments together. 3. PCR is used to sequence DNA. Which are correct? MCQ with Answer and Explanation

Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sequences. 2. DNA ligase joins DNA fragments together. 3. PCR is used to sequence DNA. Which are correct?
A. 1, 2 and 3
B. 1 and 3 only
C. 2 and 3 only
D. 1 and 2 only
Answer: Option D
Solution (By JKExamLibrary)
Restriction endonucleases are molecular scissors that recognize and cut DNA at specific, highly specific palindromic nucleotide sequences, creating fragments with sticky or blunt ends. DNA ligase is an enzyme that catalyzes the formation of phosphodiester bonds between adjacent DNA fragments, effectively joining or 'pasting' them together, crucial in recombinant DNA technology. PCR (Polymerase Chain Reaction) is used to exponentially amplify specific DNA segments, not to sequence them; DNA sequencing is a separate technique. Thus, statements 1 and 2 are correct.

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Practice More Biology Questions

Question #1
In primary ecological succession on a bare rock (xerarch), the first organisms to colonize the rock are typically:
A. Mosses
B. Grasses
C. Lichens
D. Shrubs

Correct Answer: Option C


Explanation:
In primary succession on a bare, lifeless rock (xerosere), the pioneer species are usually lichens (a symbiotic association of fungi and algae or cyanobacteria). Lichens can survive in harsh, dry conditions and secrete acids that slowly weather the rock, contributing to soil formation. Once a thin layer of soil is formed, mosses can colonize, followed by herbs, grasses, shrubs, and eventually a climax forest community.

This question belongs to: Science Biology
Question #2
Consider statements about microorganisms: 1. Bacteria can be autotrophic or heterotrophic. 2. Viruses are considered living outside a host. 3. Fungi are eukaryotic organisms. Which are correct?
A. 2 and 3 only
B. 1 and 2 only
C. 1 and 3 only
D. 1, 2 and 3

Correct Answer: Option C


Explanation:
Bacteria exhibit diverse nutritional modes; some are autotrophic (photosynthetic like cyanobacteria or chemosynthetic), while others are heterotrophic (saprophytic or parasitic). Fungi are eukaryotic organisms, characterized by having a true nucleus and membrane-bound organelles, and are heterotrophic. Viruses are considered non-living outside a host cell because they lack metabolic machinery and cannot replicate independently; they only exhibit living characteristics inside a host. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #3
The hormone that stimulates the production of red blood cells is:
A. Thrombopoietin
B. Aldosterone
C. Erythropoietin
D. Insulin

Correct Answer: Option C


Explanation:
Erythropoietin (EPO), produced mainly by the kidneys in response to low oxygen levels, stimulates the bone marrow to produce red blood cells. Insulin regulates glucose, thrombopoietin stimulates platelet production, aldosterone regulates sodium and potassium balance.

This question belongs to: Science Biology