Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct? MCQ with Answer and Explanation

Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct?
A. 2 and 3 only
B. 1 and 2 only
C. 1, 2 and 3
D. 1 and 3 only
Answer: Option B
Solution (By JKExamLibrary)
Restriction endonucleases act as molecular scissors, cleaving DNA at specific palindromic recognition sequences. Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA segments exponentially. Gel electrophoresis is primarily used to separate DNA or RNA fragments based on size, though it can separate proteins (SDS-PAGE), the statement implies it's exclusively or primarily for proteins in general biotech context, but 1 and 2 are definitively core facts. Let's refine: Gel electrophoresis separates charged molecules; for DNA it's by size. Statements 1 and 2 are universally correct core principles.

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Question #1
The structural and functional unit of heredity is the:
A. DNA
B. Nucleotide
C. Gene
D. Chromosome

Correct Answer: Option C


Explanation:
A gene is a segment of DNA that codes for a functional product (protein or RNA) and is the basic unit of heredity. It determines traits. Chromosomes are structures containing many genes. DNA is the genetic material, and nucleotides are the building blocks. Genes are passed from parents to offspring.

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Question #2
Chiasmata, the visible points of crossing over, become clearly visible during which sub-stage of Prophase I?
A. Zygotene
B. Diplotene
C. Pachytene
D. Leptotene

Correct Answer: Option B


Explanation:
Chiasmata become clearly visible during the diplotene stage of Prophase I. While the actual exchange of genetic material (crossing over) occurs during the pachytene stage, the homologous chromosomes begin to repel each other and move apart in diplotene, remaining connected only at the sites of crossing over, which appear as X-shaped chiasmata. Exam tip: Pachytene = crossing over; Diplotene = chiasmata visible.

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Question #3
Which of the following statements about tissues is correct?
A. Simple permanent tissues are made of one type of cell.
B. Xylem and phloem are examples of simple tissues.
C. Meristematic tissues are permanent tissues with thick walls.
D. Epidermis is a vascular tissue.

Correct Answer: Option A


Explanation:
Simple permanent tissues (parenchyma, collenchyma, sclerenchyma) consist of a single cell type. Meristematic tissues are dividing and have thin walls; xylem/phloem are complex; epidermis is dermal.

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