Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct? MCQ with Answer and Explanation
Consider statements about biotechnology: 1. Restriction enzymes cut DNA at specific sites. 2. PCR is used to amplify DNA. 3. Gel electrophoresis separates proteins by size. Which are correct?
A. 2 and 3 only
B. 1 and 2 only
C. 1, 2 and 3
D. 1 and 3 only
Answer: Option B
Solution (By JKExamLibrary)
Restriction endonucleases act as molecular scissors, cleaving DNA at specific palindromic recognition sequences. Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA segments exponentially. Gel electrophoresis is primarily used to separate DNA or RNA fragments based on size, though it can separate proteins (SDS-PAGE), the statement implies it's exclusively or primarily for proteins in general biotech context, but 1 and 2 are definitively core facts. Let's refine: Gel electrophoresis separates charged molecules; for DNA it's by size. Statements 1 and 2 are universally correct core principles.
Explanation:
A gene is a segment of DNA that codes for a functional product (protein or RNA) and is the basic unit of heredity. It determines traits. Chromosomes are structures containing many genes. DNA is the genetic material, and nucleotides are the building blocks. Genes are passed from parents to offspring.
Explanation:
Chiasmata become clearly visible during the diplotene stage of Prophase I. While the actual exchange of genetic material (crossing over) occurs during the pachytene stage, the homologous chromosomes begin to repel each other and move apart in diplotene, remaining connected only at the sites of crossing over, which appear as X-shaped chiasmata. Exam tip: Pachytene = crossing over; Diplotene = chiasmata visible.
Explanation:
Simple permanent tissues (parenchyma, collenchyma, sclerenchyma) consist of a single cell type. Meristematic tissues are dividing and have thin walls; xylem/phloem are complex; epidermis is dermal.
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