In biotechnology, the 'PCR' (Polymerase Chain Reaction) technique requires a thermostable DNA polymerase. The most commonly used enzyme for this is: MCQ with Answer and Explanation

In biotechnology, the 'PCR' (Polymerase Chain Reaction) technique requires a thermostable DNA polymerase. The most commonly used enzyme for this is:
A. Reverse transcriptase
B. Taq polymerase
C. T7 polymerase
D. DNA polymerase I
Answer: Option B
Solution (By JKExamLibrary)
PCR involves repeated cycles of high-temperature denaturation (around 94°C) to separate the DNA strands. Normal DNA polymerases would be destroyed at these temperatures. Therefore, a thermostable DNA polymerase is required. Taq polymerase, isolated from the thermophilic bacterium Thermus aquaticus found in hot springs, is the most commonly used enzyme because it can withstand the high denaturation temperatures without losing its activity, eliminating the need to add fresh enzyme after each cycle.

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What is the correct sequence of taxonomic hierarchy from highest to lowest?
A. Kingdom, Class, Phylum, Order, Family, Genus, Species
B. Kingdom, Family, Class, Order, Phylum, Genus, Species
C. Kingdom, Phylum, Class, Order, Family, Genus, Species
D. Kingdom, Phylum, Order, Class, Family, Genus, Species

Correct Answer: Option C


Explanation:
The correct hierarchical classification of organisms, from the broadest to the most specific category, is: Kingdom, Phylum (or Division for plants), Class, Order, Family, Genus, and Species. A helpful mnemonic to remember this is: 'King Philip Came Over For Good Spaghetti'.

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The scientist who first used the term 'antibiotic' was:
A. Paul Ehrlich
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C. Gerhard Domagk
D. Alexander Fleming

Correct Answer: Option B


Explanation:
Selman Waksman coined the term 'antibiotic' in 1942 to describe chemical compounds produced by microorganisms that inhibit or kill other microbes. Fleming discovered penicillin, Ehrlich developed the concept of chemotherapy, Domagk discovered sulfa drugs.

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Correct Answer: Option B


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