The restriction enzyme EcoRI recognizes the palindromic DNA sequence 5'-GAATTC-3' and cuts the DNA between the: MCQ with Answer and Explanation

The restriction enzyme EcoRI recognizes the palindromic DNA sequence 5'-GAATTC-3' and cuts the DNA between the:
A. A and T
B. T and C
C. G and A
D. C and G
Answer: Option C
Solution (By JKExamLibrary)
Restriction enzymes are molecular scissors that cut DNA at specific recognition sites. EcoRI, isolated from Escherichia coli strain RY13, recognizes the 6-base pair palindromic sequence 5'-GAATTC-3'. It cuts the phosphodiester bond between the G and the A on both strands, leaving single-stranded overhangs called 'sticky ends' (5'-AATT-3'). These sticky ends are crucial for joining DNA fragments from different sources using DNA ligase.

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