The technique of separating molecules based on their size and charge is: MCQ with Answer and Explanation

The technique of separating molecules based on their size and charge is:
A. Chromatography
B. Electrophoresis
C. Spectrophotometry
D. Centrifugation
Answer: Option B
Solution (By JKExamLibrary)
Gel electrophoresis, especially agarose gel electrophoresis, separates DNA, RNA, or protein fragments based on size and charge under an electric field. Smaller fragments move faster through the gel matrix. Centrifugation separates by density, chromatography by differential partitioning, spectrophotometry measures light absorption. Electrophoresis is a fundamental tool in molecular biology.

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Practice More Biology Questions

Question #1
Consider the following statements about proteins:
1. They are made of amino acids.
2. Peptide bonds link amino acids.
3. Hemoglobin is a transport protein.
Which of the above is/are correct?
A. 1, 2, and 3
B. 2 and 3 only
C. 1 and 3 only
D. 1 and 2 only

Correct Answer: Option A


Explanation:
All are correct: proteins are polymers of amino acids joined by peptide bonds; hemoglobin is a protein that transports oxygen in blood.

This question belongs to: Science Biology
Question #2
Phagocytosis is a type of which cellular process?
A. Plasmolysis
B. Endocytosis
C. Osmosis
D. Exocytosis

Correct Answer: Option B


Explanation:
Phagocytosis, or 'cell eating', is a type of endocytosis where the cell membrane engulfs large solid particles, such as bacteria or cellular debris, forming a vesicle called a phagosome. This phagosome then fuses with a lysosome for digestion. It is primarily performed by specialized cells like macrophages and neutrophils.

This question belongs to: Science Biology
Question #3
Which of the following vitamins is a water-soluble vitamin that acts as a coenzyme in the metabolism of amino acids and fatty acids?
A. Vitamin A
B. Vitamin D
C. Vitamin B6 (Pyridoxine)
D. Vitamin K

Correct Answer: Option C


Explanation:
Vitamin B6, in its active form pyridoxal phosphate (PLP), is a crucial water-soluble coenzyme involved in over 100 enzymatic reactions, primarily in amino acid metabolism (transamination, decarboxylation) and fatty acid metabolism. It is also essential for neurotransmitter synthesis and hemoglobin formation. Vitamins A, D, and K are fat-soluble vitamins. Exam tip: B-complex and C are water-soluble; A, D, E, K are fat-soluble.

This question belongs to: Science Biology