The technique of using radioactive probes to detect specific DNA sequences on a membrane is:
A. Flow cytometry
B. Southern blotting
C. ELISA
D. PCR
Answer: Option B
Solution (By JKExamLibrary)
Southern blotting involves separation of DNA fragments by gel electrophoresis, transfer to a membrane, and hybridization with a labeled probe. PCR amplifies DNA, ELISA detects proteins, flow cytometry analyzes cells.
Explanation:
Abscission is the controlled shedding of leaves, flowers, or fruits, facilitated by the hormone ethylene and enzymes that degrade cell walls at the abscission zone. Senescence is the aging process leading to cell death. Transpiration is water loss, germination is seed sprouting.
Explanation:
Pellagra, characterized by dermatitis, diarrhea, and dementia (3 Ds), is due to deficiency of niacin (vitamin B₃) or its precursor tryptophan. Niacin is part of NAD and NADP coenzymes.
Explanation:
Penicillin targets the transpeptidase enzyme, blocking peptidoglycan cross-linking, leading to cell lysis. This is the mechanism, so R correctly explains A.
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