The technique used to amplify DNA in a test tube is: MCQ with Answer and Explanation

The technique used to amplify DNA in a test tube is:
A. Southern blotting
B. Gel electrophoresis
C. Sequencing
D. PCR
Answer: Option D
Solution (By JKExamLibrary)
Polymerase chain reaction (PCR) uses Taq polymerase, primers, and thermal cycling to make millions of copies of a specific DNA sequence. Southern blotting detects DNA, electrophoresis separates, sequencing reads bases.

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Practice More Biology Questions

Question #1
Which class of antibody is the most abundant in human blood plasma?
A. IgA
B. IgE
C. IgM
D. IgG

Correct Answer: Option D


Explanation:
Immunoglobulin G (IgG) is the most abundant antibody in human blood plasma, constituting about 75-80% of all antibodies. It is the only antibody that can cross the placenta, providing passive immunity to the fetus. IgG is crucial for long-term immunity, neutralizing toxins, viruses, and bacteria, and activating the complement system.

This question belongs to: Science Biology
Question #2
Consider statements about cell division: 1. Cytokinesis in plant cells occurs by cell plate formation. 2. Meiosis maintains the chromosome number. 3. Mitosis occurs in somatic cells. Which are correct?
A. 1 and 2 only
B. 1, 2 and 3
C. 2 and 3 only
D. 1 and 3 only

Correct Answer: Option D


Explanation:
In plant cells, which have rigid cell walls, cytokinesis occurs by the formation of a new cell wall (cell plate) in the middle of the cell. Mitosis is equational division occurring in somatic cells for growth and repair, maintaining the same chromosome number. Meiosis is reductional division, halving the chromosome number to produce gametes. Thus, statements 1 and 3 are correct.

This question belongs to: Science Biology
Question #3
The hormone that increases blood calcium levels is:
A. Thyroxine
B. Calcitonin
C. Parathyroid hormone
D. Insulin

Correct Answer: Option C


Explanation:
Parathyroid hormone (PTH) raises blood calcium by increasing bone resorption, kidney reabsorption, and activating vitamin D. Calcitonin lowers calcium. Thyroxine regulates metabolism, insulin glucose.

This question belongs to: Science Biology