The technique used to detect specific proteins in a tissue section using labeled antibodies is: MCQ with Answer and Explanation

The technique used to detect specific proteins in a tissue section using labeled antibodies is:
A. Flow cytometry
B. Western blotting
C. ELISA
D. Immunohistochemistry
Answer: Option D
Solution (By JKExamLibrary)
Immunohistochemistry (IHC) uses enzyme- or fluorophore-linked antibodies to visualize specific antigens in tissue sections. ELISA is for liquid samples, Western blot for membrane, flow cytometry for cells.

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Practice More Biology Questions

Question #1
In plant physiology, the 'Munch hypothesis' for the translocation of sugars in the phloem relies on the creation of a:
A. Turgor pressure gradient
B. Cohesion-tension force
C. Transpiration pull
D. Root pressure

Correct Answer: Option A


Explanation:
The Munch hypothesis, or mass flow hypothesis, explains the movement of sugars (primarily sucrose) through the phloem. It proposes that sugars are actively loaded into the phloem at the source (e.g., leaves), which lowers the water potential and causes water to enter from the xylem by osmosis. This creates a high turgor pressure. At the sink (e.g., roots, fruits), sugars are unloaded, increasing water potential, and water leaves the phloem, creating a low turgor pressure. The bulk flow of sap is driven by this turgor pressure gradient from source to sink.

This question belongs to: Science Biology
Question #2
The release of which hormone is stimulated by the suckling reflex during breastfeeding?
A. Oxytocin
B. Prolactin
C. Estrogen
D. Progesterone

Correct Answer: Option A


Explanation:
Oxytocin, produced in the hypothalamus and released from the posterior pituitary, triggers the milk let-down reflex by causing myoepithelial cells around alveoli to contract upon suckling. Prolactin stimulates milk synthesis. Estrogen and progesterone are involved in breast development during pregnancy but not the immediate ejection reflex.

This question belongs to: Science Biology
Question #3
Consider statements about the nucleus: 1. It is bounded by a single membrane. 2. It contains the nucleolus. 3. Nuclear pores allow RNA export. Which are correct?
A. 1 and 2 only
B. 2 and 3 only
C. 1 and 3 only
D. 1, 2 and 3

Correct Answer: Option B


Explanation:
The nucleus is bounded by a double membrane called the nuclear envelope, not a single membrane. It houses the nucleolus, which is the site of ribosomal RNA synthesis. The nuclear envelope is perforated by nuclear pores that regulate the bidirectional transport of molecules, including the export of mRNA to the cytoplasm. Thus, statements 2 and 3 are correct.

This question belongs to: Science Biology