The technique used to determine the nucleotide sequence of DNA is: MCQ with Answer and Explanation

The technique used to determine the nucleotide sequence of DNA is:
A. Gel electrophoresis
B. DNA sequencing
C. PCR
D. Southern blotting
Answer: Option B
Solution (By JKExamLibrary)
DNA sequencing determines the precise order of nucleotides in a DNA fragment. Sanger's dideoxy chain-termination method and next-generation sequencing are common. PCR amplifies DNA, gel electrophoresis separates by size, Southern blotting detects specific sequences. Sequencing is fundamental in genomics.

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Practice More Biology Questions

Question #1
Consider statements about animal husbandry: 1. Dairy farming involves the rearing of milk-producing animals. 2. Poultry farming involves the rearing of birds for meat and eggs. 3. Apiculture involves the rearing of silkworms. Which are correct?
A. 1 and 2 only
B. 2 and 3 only
C. 1 and 3 only
D. 1, 2 and 3

Correct Answer: Option A


Explanation:
Dairy farming is the business of breeding, raising, and managing dairy animals (like cows and buffaloes) for the production of milk and milk products. Poultry farming involves the commercial raising of domesticated birds (like chickens, ducks, and turkeys) for the production of meat and eggs. Apiculture is the scientific rearing of honeybees (Apis species) for the production of honey, beeswax, and other hive products; silkworm rearing is sericulture. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #2
Consider statements about the endocrine system: 1. The pituitary gland is called the master gland. 2. The thyroid gland secretes calcitonin. 3. The adrenal medulla secretes cortisol. Which are correct?
A. 1 and 3 only
B. 1 and 2 only
C. 2 and 3 only
D. 1, 2 and 3

Correct Answer: Option B


Explanation:
The pituitary gland is termed the master gland because it secretes hormones that regulate other endocrine glands. The thyroid gland secretes thyroxine, triiodothyronine, and calcitonin, which regulates blood calcium. The adrenal cortex secretes cortisol (a glucocorticoid), while the adrenal medulla secretes adrenaline and noradrenaline. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #3
The type of plant that has adapted to survive in very dry environments is called:
A. Mesophyte
B. Halophyte
C. Xerophyte
D. Hydrophyte

Correct Answer: Option C


Explanation:
Xerophytes (e.g., cactus, acacia) have adaptations like thick cuticle, sunken stomata, reduced leaves, and water-storing tissues to conserve water in arid habitats. Hydrophytes live in water, mesophytes in moderate conditions, halophytes in saline soils. Xerophytes minimize transpiration and maximize water uptake.

This question belongs to: Science Biology