The technique used to produce a large number of identical copies of a specific DNA fragment is: MCQ with Answer and Explanation

The technique used to produce a large number of identical copies of a specific DNA fragment is:
A. Gel electrophoresis
B. DNA sequencing
C. Microarray
D. PCR
Answer: Option D
Solution (By JKExamLibrary)
Polymerase chain reaction (PCR) amplifies a specific DNA segment exponentially using primers, DNA polymerase (Taq), and thermal cycling. Gel electrophoresis separates DNA by size, sequencing determines base order, microarray analyzes gene expression. PCR is crucial in cloning, diagnostics, and forensics.

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Practice More Biology Questions

Question #1
Typhoid fever is caused by which bacterium?
A. Vibrio cholerae
B. Mycobacterium leprae
C. Salmonella typhi
D. Streptococcus pneumoniae

Correct Answer: Option C


Explanation:
Typhoid fever is a life-threatening systemic infection caused by the bacterium Salmonella typhi. It is transmitted through contaminated food and water. Symptoms include sustained high fever, headache, abdominal pain, and sometimes a rash. The Widal test is a common diagnostic test for typhoid. S. pneumoniae causes pneumonia, and V. cholerae causes cholera.

This question belongs to: Science Biology
Question #2
Glycogen is a polysaccharide primarily stored in:
A. Bacterial cell membranes
B. Animal liver and muscle cells
C. Plant cells
D. Fungal cell walls

Correct Answer: Option B


Explanation:
Glycogen is a highly branched polysaccharide of glucose, often referred to as 'animal starch'. It is the primary storage form of carbohydrates in animals and fungi. It is mainly stored in the liver and muscle cells. When blood glucose levels drop, glycogen is broken down (glycogenolysis) to release glucose for energy.

This question belongs to: Science Biology
Question #3
Assertion (A): The cell wall of plants is freely permeable.
Reason (R): It allows water and dissolved substances to pass through.
A. Both A and R are true, but R is not the correct explanation.
B. Both A and R are true, and R is the correct explanation.
C. A true, R false.
D. A false, R true.

Correct Answer: Option B


Explanation:
The cell wall is porous and does not regulate entry; the plasma membrane is selectively permeable. Thus, the wall is freely permeable, and the reason explains it.

This question belongs to: Science Biology