A plasmid used as a cloning vector must have: MCQ with Answer and Explanation

A plasmid used as a cloning vector must have:
A. An origin of replication and a selectable marker
B. No antibiotic resistance genes
C. A large size to hold more DNA
D. A single restriction site only
Answer: Option A
Solution (By JKExamLibrary)
A plasmid used as a cloning vector in genetic engineering must have certain essential features: an Origin of Replication (ori) to ensure its replication within the host cell, a Selectable Marker (like an antibiotic resistance gene) to identify and eliminate non-transformants, and at least one unique Restriction Site (cloning site) for the insertion of the foreign DNA.

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Practice More Biology Questions

Question #1
The organ that produces bile is the:
A. Gall bladder
B. Pancreas
C. Liver
D. Duodenum

Correct Answer: Option C


Explanation:
Bile is continuously synthesized by hepatocytes in the liver. It contains bile salts, bile pigments, cholesterol, and phospholipids. The gall bladder merely stores and concentrates bile, releasing it upon eating. The pancreas produces digestive enzymes, duodenum is part of the small intestine. Bile emulsifies fats.

This question belongs to: Science Biology
Question #2
Consider statements about genetics: 1. DNA contains the sugar deoxyribose. 2. RNA contains the sugar ribose. 3. DNA is single-stranded. Which are correct?
A. 1 and 3 only
B. 2 and 3 only
C. 1 and 2 only
D. 1, 2 and 3

Correct Answer: Option C


Explanation:
DNA (deoxyribonucleic acid) is characterized by the presence of the pentose sugar deoxyribose in its nucleotide backbone. RNA (ribonucleic acid) contains the pentose sugar ribose. DNA is typically a double-stranded helical molecule, providing stability for long-term genetic storage. RNA is generally single-stranded, allowing it to fold into complex shapes and perform various functional roles like mRNA, tRNA, and rRNA. Thus, statements 1 and 2 are correct.

This question belongs to: Science Biology
Question #3
The type of reproduction that involves only one parent and no gamete fusion is:
A. Asexual reproduction
B. Fertilization
C. Cross-pollination
D. Sexual reproduction

Correct Answer: Option A


Explanation:
Asexual reproduction (binary fission, budding, fragmentation, vegetative propagation) produces genetically identical offspring from a single parent. Sexual reproduction involves gamete fusion. Fertilization is gamete union.

This question belongs to: Science Biology