The enzyme Taq polymerase, used in PCR, is obtained from: MCQ with Answer and Explanation

The enzyme Taq polymerase, used in PCR, is obtained from:
A. Agrobacterium tumefaciens
B. Escherichia coli
C. Salmonella typhimurium
D. Thermus aquaticus
Answer: Option D
Solution (By JKExamLibrary)
Taq polymerase is a thermostable DNA polymerase enzyme isolated from the thermophilic bacterium Thermus aquaticus, found in hot springs. It is used in the Polymerase Chain Reaction (PCR) because it can withstand the high temperatures (around 94°C) required for the denaturation step of PCR without being destroyed, unlike normal DNA polymerases.

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The process of converting mRNA information into a polypeptide chain is:
A. Transcription
B. Transduction
C. Replication
D. Translation

Correct Answer: Option D


Explanation:
Translation is the synthesis of a polypeptide chain at the ribosome, where the genetic code in mRNA is read by tRNA anticodons to assemble amino acids in the correct order. Transcription produces mRNA from DNA, replication duplicates DNA, transduction is gene transfer by viruses. Translation involves initiation, elongation, and termination factors.

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Which of the following is a nitrogen-fixing bacterium in legume roots?
A. Azotobacter
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Correct Answer: Option C


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Rhizobium species form symbiotic root nodules in legumes and fix atmospheric nitrogen into ammonia. Azotobacter is free-living, Nitrosomonas nitrifies, Lactobacillus ferments.

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Which type of joint is present between the skull bones?
A. Fibrous joint (suture)
B. Ball and socket joint
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Correct Answer: Option A


Explanation:
The joints between skull bones are immovable fibrous joints called sutures, where bones are tightly connected by dense connective tissue. Hinge joint (elbow, knee) allows movement in one plane. Ball and socket (shoulder, hip) allows multi-directional movement. Pivot joint (atlas-axis) allows rotational movement. Sutures fuse completely in old age.

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