The enzyme used to join DNA fragments in genetic engineering is: MCQ with Answer and Explanation

The enzyme used to join DNA fragments in genetic engineering is:
A. DNA ligase
B. Helicase
C. Restriction enzyme
D. DNA polymerase
Answer: Option A
Solution (By JKExamLibrary)
DNA ligase seals the phosphodiester bonds between adjacent DNA fragments, joining them together. In recombinant DNA technology, it is used to ligate the gene of interest into a vector. Restriction enzymes cut DNA, DNA polymerase synthesizes DNA, and helicase unwinds the double helix. Ligase is essential for finalizing the recombinant DNA molecule.

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Which of the following diseases is caused by a protozoan transmitted by the tsetse fly?
A. Yellow fever
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C. Sleeping sickness
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Correct Answer: Option C


Explanation:
African sleeping sickness (trypanosomiasis) is caused by the protozoan Trypanosoma brucei, transmitted by the tsetse fly (Glossina). Malaria is by Plasmodium (Anopheles mosquito), filariasis by Wuchereria (Culex), yellow fever by a virus (Aedes). Sleeping sickness affects the central nervous system, causing lethargy, coma, and death if untreated.

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Question #2
The structure that carries oxygenated blood from the lungs to the heart is the:
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Correct Answer: Option B


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The process by which a bacterial cell takes up DNA from its environment is:
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Correct Answer: Option C


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Transformation is the direct uptake and incorporation of naked DNA from the surroundings by a bacterium. Conjugation is DNA transfer via pilus, transduction via phage, lysogeny is phage DNA integration.

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