The process of introducing DNA into bacterial cells by applying a brief electrical pulse is: MCQ with Answer and Explanation

The process of introducing DNA into bacterial cells by applying a brief electrical pulse is:
A. Electroporation
B. Heat shock
C. Biolistics
D. Microinjection
Answer: Option A
Solution (By JKExamLibrary)
Electroporation uses an electric field to create temporary pores in the cell membrane, allowing DNA to enter. Heat shock is a chemical method using CaCl₂ and temperature shift, microinjection physically injects DNA into cells, biolistics is a gene gun for plant cells. Electroporation is efficient for many cell types.

Discuss this Question (0)

No comments yet. Be the first to start the discussion!

Practice More Biology Questions

Question #1
The microorganism responsible for the leavening of bread dough is:
A. Aspergillus
B. Propionibacterium
C. Saccharomyces cerevisiae (Yeast)
D. Lactobacillus

Correct Answer: Option C


Explanation:
The leavening (rising) of bread dough is caused by the anaerobic respiration (fermentation) of the yeast Saccharomyces cerevisiae (baker's yeast). The yeast ferments the sugars in the dough, producing carbon dioxide (CO2) gas and ethanol. The CO2 gas gets trapped in the dough, causing it to expand and rise, giving bread its light and fluffy texture.

This question belongs to: Science Biology
Question #2
National parks and wildlife sanctuaries are examples of which type of conservation?
A. Ex-situ conservation
B. In-situ conservation
C. Tissue culture
D. Cryopreservation

Correct Answer: Option B


Explanation:
In-situ (on-site) conservation involves the conservation of species in their natural habitats. National parks, wildlife sanctuaries, and biosphere reserves are examples of in-situ conservation. Ex-situ (off-site) conservation involves taking endangered species out of their natural habitat and placing them in special settings like zoological parks, botanical gardens, and seed banks for conservation and breeding.

This question belongs to: Science Biology
Question #3
Assertion (A): The cell wall of fungi is made of cellulose.
Reason (R): Chitin is a characteristic component of fungal cell walls.
A. A is true, but R is false.
B. Both A and R are true, and R is the correct explanation.
C. A is false, but R is true.
D. Both A and R are true, but R is not the correct explanation.

Correct Answer: Option C


Explanation:
Fungal cell walls contain chitin, not cellulose. Cellulose is found in plant cell walls. Thus, A is false and R is true.

This question belongs to: Science Biology