The process of 'Southern blotting' is a technique used in molecular biology to detect: MCQ with Answer and Explanation

The process of 'Southern blotting' is a technique used in molecular biology to detect:
A. Lipids in a cell membrane
B. Specific DNA sequences in a sample
C. Specific proteins in a sample
D. Specific RNA sequences in a sample
Answer: Option B
Solution (By JKExamLibrary)
Southern blotting, named after its inventor Edwin Southern, is a laboratory method used to detect specific DNA sequences in a complex DNA sample. The process involves digesting DNA with restriction enzymes, separating the fragments by gel electrophoresis, transferring (blotting) them onto a nylon or nitrocellulose membrane, and then hybridizing the membrane with a labeled, complementary DNA or RNA probe. Northern blotting detects RNA, and Western blotting detects proteins.

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Practice More Biology Questions

Question #1
The fluid mosaic model describes the structure of:
A. Plasma membrane
B. Nucleus
C. Cell wall
D. Cytoplasm

Correct Answer: Option A


Explanation:
The fluid mosaic model (Singer-Nicolson) depicts the cell membrane as a phospholipid bilayer with embedded proteins, carbohydrates, and cholesterol, giving it fluidity.

This question belongs to: Science Biology
Question #2
The animal that exhibits 'canine' teeth adapted for tearing flesh is characteristic of:
A. Omnivores
B. Carnivores
C. Detritivores
D. Herbivores

Correct Answer: Option B


Explanation:
Carnivores have well-developed, sharp, pointed canines for piercing and tearing flesh. Herbivores have reduced or absent canines, with well-developed molars for grinding. Omnivores like humans have moderate canines. Detritivores feed on detritus. Dental formula and tooth type are adaptations to diet. Dogs, cats, and lions have prominent canines.

This question belongs to: Science Biology
Question #3
The structure that provides buoyancy to aquatic plants is:
A. Collenchyma
B. Aerenchyma
C. Sclerenchyma
D. Xylem

Correct Answer: Option B


Explanation:
Aerenchyma is a specialized parenchyma tissue with large intercellular air spaces, providing buoyancy and facilitating gas exchange in aquatic plants. It also helps in oxygen transport to submerged roots. Sclerenchyma is supportive, collenchyma flexible support, xylem water-conducting.

This question belongs to: Science Biology