The technique used to amplify small quantities of DNA in vitro is: MCQ with Answer and Explanation

The technique used to amplify small quantities of DNA in vitro is:
A. DNA sequencing
B. Southern blotting
C. Gel electrophoresis
D. Polymerase chain reaction (PCR)
Answer: Option D
Solution (By JKExamLibrary)
PCR, invented by Kary Mullis, allows exponential amplification of a specific DNA segment using a thermostable DNA polymerase (Taq polymerase), primers, and thermal cycling. Gel electrophoresis separates DNA fragments, Southern blotting detects specific sequences, DNA sequencing determines the order of bases. PCR is widely used in diagnostics, forensics, and research.

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Practice More Biology Questions

Question #1
The technique used to introduce foreign DNA into cells by applying a brief electric pulse is:
A. Microinjection
B. Biolistics
C. Lipofection
D. Electroporation

Correct Answer: Option D


Explanation:
Electroporation creates transient pores in the cell membrane using an electric field, allowing DNA to enter. Microinjection uses a fine needle, biolistics a gene gun, lipofection lipid vesicles.

This question belongs to: Science Biology
Question #2
The normal systolic blood pressure of a healthy adult is about:
A. 80 mmHg
B. 120 mmHg
C. 160 mmHg
D. 200 mmHg

Correct Answer: Option B


Explanation:
Normal blood pressure is around 120/80 mmHg (systolic/diastolic). Systolic pressure is during ventricular contraction; diastolic during relaxation. Hypertension is >140/90 mmHg.

This question belongs to: Science Biology
Question #3
The 'Spleen' in the human body is primarily located in the:
A. Pelvic cavity
B. Abdominal cavity, in the upper left quadrant
C. Thoracic cavity
D. Cranial cavity

Correct Answer: Option B


Explanation:
The spleen is the largest lymphoid organ in the body. It is located in the abdominal cavity, specifically in the upper left quadrant, tucked between the stomach and the diaphragm, protected by the rib cage (ribs 9-11). It functions primarily in filtering blood, removing old or damaged red blood cells, storing platelets, and mounting immune responses against blood-borne pathogens. It is not located in the thoracic, pelvic, or cranial cavities.

This question belongs to: Science Biology