The pigment that absorbs light energy for photosynthesis is mainly located in the: MCQ with Answer and Explanation

Explanation:
DNA ligase seals the phosphodiester bonds between adjacent DNA fragments, joining them together. In recombinant DNA technology, it is used to ligate the gene of interest into a vector. Restriction enzymes cut DNA, DNA polymerase synthesizes DNA, and helicase unwinds the double helix. Ligase is essential for finalizing the recombinant DNA molecule.

Explanation:
The Law of Segregation (Mendel's First Law) states that an organism possesses two alleles for each trait, and these alleles separate (segregate) during the formation of gametes (meiosis). Each gamete receives only one allele. This explains why a recessive trait can disappear in the F1 generation but reappear in the F2 generation.

Explanation:
Restriction endonucleases act as molecular scissors, cleaving DNA at specific palindromic recognition sequences. Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA segments exponentially. Gel electrophoresis is primarily used to separate DNA or RNA fragments based on size, though it can separate proteins (SDS-PAGE), the statement implies it's exclusively or primarily for proteins in general biotech context, but 1 and 2 are definitively core facts. Let's refine: Gel electrophoresis separates charged molecules; for DNA it's by size. Statements 1 and 2 are universally correct core principles.