The method of separating proteins based on their size using an electric field is: MCQ with Answer and Explanation

The method of separating proteins based on their size using an electric field is:
A. DNA sequencing
B. ELISA
C. SDS-PAGE
D. PCR
Answer: Option C
Solution (By JKExamLibrary)
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) denatures proteins and coats them with negative charge, allowing separation by molecular weight in an electric field. PCR amplifies DNA, sequencing determines base order, ELISA detects antigens/antibodies. SDS-PAGE is a fundamental biochemistry technique.

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Practice More Biology Questions

Question #1
The transgenic cow 'Rosie' was known for producing milk enriched with:
A. Human alpha-lactalbumin
B. Antithrombin
C. Human insulin
D. Bovine growth hormone

Correct Answer: Option A


Explanation:
Rosie, the first transgenic cow, produced milk containing human alpha-lactalbumin, a protein important for infant nutrition. This was achieved by introducing the human gene for the protein into the cow. Human insulin is produced in E. coli or yeast, growth hormone in some transgenic animals, and antithrombin in goat milk (ATryn). Transgenic animals are used as bioreactors.

This question belongs to: Science Biology
Question #2
The vitamin that acts as a coenzyme in the synthesis of DNA and red blood cells is:
A. Vitamin C
B. Vitamin K
C. Vitamin A
D. Vitamin B₁₂

Correct Answer: Option D


Explanation:
Vitamin B₁₂ (cobalamin) is required for DNA synthesis and RBC maturation. Deficiency causes pernicious anemia. It also maintains myelin sheath.

This question belongs to: Science Biology
Question #3
Consider statements about the human nervous system: 1. The brain is protected by the cranium. 2. The spinal cord is protected by the vertebral column. 3. Neurons are non-excitable cells. Which are correct?
A. 2 and 3 only
B. 1 and 2 only
C. 1 and 3 only
D. 1, 2 and 3

Correct Answer: Option B


Explanation:
The central nervous system comprises the brain and spinal cord, protected by the bony cranium and vertebral column, respectively, along with meninges. Neurons are highly excitable cells capable of generating and conducting action potentials in response to stimuli. Therefore, statements 1 and 2 are correct, while statement 3 is fundamentally incorrect.

This question belongs to: Science Biology