The technique used to separate DNA fragments based on size is:
A. Cloning
B. DNA sequencing
C. Gel electrophoresis
D. PCR
Answer: Option C
Solution (By JKExamLibrary)
Agarose gel electrophoresis separates DNA fragments by size under an electric field; smaller fragments migrate faster. PCR amplifies DNA, sequencing determines base order, cloning produces copies.
Explanation:
Urine specific gravity, a measure of solute concentration, normally ranges from 1.015 to 1.030. It varies with hydration status: low when dilute (after drinking), high when concentrated (dehydration). Diabetes insipidus shows low SG, diabetes mellitus may show high due to glucose.
Explanation:
Glucagon, secreted by alpha cells of the pancreas when blood glucose is low, binds to liver receptors activating glycogenolysis (glycogen → glucose) and gluconeogenesis. Insulin promotes glycogenesis (glucose → glycogen). Thyroxine affects metabolism, somatostatin inhibits both insulin and glucagon.
Explanation:
Certain bacteria, like Bacillus megaterium and Alcaligenes eutrophus, accumulate polyhydroxyalkanoates (PHA) such as PHB as intracellular carbon and energy storage under nutrient stress. These are used to make biodegradable plastics. E. coli is engineered for some bioplastics, but natural producers include Bacillus.
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