The technique used to measure the amount of light absorbed by a substance is: MCQ with Answer and Explanation

The technique used to measure the amount of light absorbed by a substance is:
A. Spectrophotometry
B. Electrophoresis
C. Centrifugation
D. Chromatography
Answer: Option A
Solution (By JKExamLibrary)
Spectrophotometry quantifies the concentration of a substance by measuring the absorbance of light at specific wavelengths. Chromatography separates mixtures, electrophoresis separates charged molecules, centrifugation separates by density/size. Spectrophotometers are widely used in biochemical and clinical labs.

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Practice More Biology Questions

Question #1
The pigment present in rod cells of the retina that is responsible for vision in dim light is:
A. Hemoglobin
B. Melanin
C. Rhodopsin
D. Iodopsin

Correct Answer: Option C


Explanation:
Rhodopsin (visual purple) is a photopigment in rod cells consisting of opsin and retinal (from vitamin A). It bleaches upon absorbing light, initiating signal transduction for vision in low light. Cone cells contain iodopsin for color vision. Melanin is skin pigment, hemoglobin carries oxygen.

This question belongs to: Science Biology
Question #2
The process of converting atmospheric nitrogen into ammonia by microorganisms is called:
A. Denitrification
B. Nitrogen fixation
C. Ammonification
D. Nitrification

Correct Answer: Option B


Explanation:
Biological nitrogen fixation, performed by bacteria (Rhizobium, Azotobacter) and cyanobacteria, reduces N₂ to NH₃. Nitrification oxidizes ammonia to nitrates, denitrification returns N₂, ammonification releases ammonia from organic matter.

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Question #3
In the male reproductive system, the spermatozoa are stored and mature in the:
A. Seminal vesicles
B. Epididymis
C. Vas deferens
D. Prostate gland

Correct Answer: Option B


Explanation:
The epididymis is a coiled tube on the posterior surface of the testis where spermatozoa undergo maturation and are stored until ejaculation. Sperm acquire motility here. Seminal vesicles and prostate gland secrete seminal fluid. Vas deferens transports sperm from epididymis to ejaculatory duct. Testes produce sperm and testosterone. Sperm maturation includes gaining ability to fertilize.

This question belongs to: Science Biology