The technique used to produce monoclonal antibodies involves fusion of B cells with: MCQ with Answer and Explanation

Explanation:
HIV is a retrovirus with two copies of single-stranded RNA as its genome. It uses reverse transcriptase to convert RNA into DNA upon infection. Double-stranded DNA is typical of many organisms. Single-stranded DNA viruses exist (e.g., parvovirus). Double-stranded RNA viruses include rotavirus. The viral RNA genome enables integration into host DNA via a DNA intermediate.

Explanation:
O negative blood group lacks A and B antigens on RBCs and Rh antigen. Hence, it can be transfused to any person without causing agglutination, making it the universal donor. AB positive has both antigens and is the universal recipient. Blood grouping is based on ABO and Rh systems. O negative is crucial in emergency transfusions when blood type is unknown.

Explanation:
Gel electrophoresis is a widely used technique to separate charged molecules, primarily DNA, RNA, or proteins, in an electric field through a gel matrix (usually agarose for DNA). DNA fragments are separated based on their size (length in base pairs). Smaller fragments move faster and further through the gel than larger ones. The separated DNA bands are visualized using ethidium bromide and UV light.