The technique used to separate proteins based on their isoelectric point is:
A. PCR
B. Isoelectric focusing
C. SDS-PAGE
D. Western blotting
Answer: Option B
Solution (By JKExamLibrary)
Isoelectric focusing (IEF) separates proteins in a pH gradient according to their isoelectric point (pI), the pH at which a protein has no net charge and stops migrating. SDS-PAGE separates by size, Western blotting detects specific proteins after separation, PCR amplifies DNA. IEF is often used in 2D gel electrophoresis.
Consider statements about nutrition: 1. Kwashiorkor is caused by protein deficiency. 2. Marasmus is caused by overall calorie deficiency. 3. Goiter is caused by iron deficiency. Which are correct?
Explanation:
Kwashiorkor is a severe form of malnutrition caused by inadequate protein intake despite sufficient calorie consumption. Marasmus results from a severe deficiency of both calories and protein, leading to extreme wasting. Goiter is the enlargement of the thyroid gland caused by iodine deficiency, not iron deficiency (which causes anemia). Thus, statements 1 and 2 are correct.
Explanation:
The pineal gland, located in the brain, secretes melatonin, which regulates circadian rhythms and the sleep-wake cycle. Its secretion increases in darkness.
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