The disease caused by the bacterium Corynebacterium diphtheriae is: MCQ with Answer and Explanation

Explanation:
The Bohr effect describes how an increase in the partial pressure of CO2 (pCO2) or a decrease in pH (increase in H+ concentration) reduces hemoglobin's affinity for oxygen, shifting the oxygen dissociation curve to the right. This facilitates the unloading of oxygen in metabolically active tissues where CO2 and acid levels are high. The reverse occurs in the lungs, enhancing oxygen loading.

Explanation:
Minamata disease is a severe neurological syndrome caused by severe mercury poisoning. It was first discovered in Minamata city, Kumamoto prefecture, Japan, in 1956. It was caused by the release of methylmercury in the industrial wastewater from the Chisso Corporation's chemical factory, which contaminated the local bay and the fish and shellfish. People who consumed the contaminated seafood suffered from severe neurological damage, including ataxia, numbness, and muscle weakness.

Explanation:
Isoelectric focusing (IEF) separates proteins in a pH gradient according to their isoelectric point (pI), the pH at which a protein has no net charge and stops migrating. SDS-PAGE separates by size, Western blotting detects specific proteins after separation, PCR amplifies DNA. IEF is often used in 2D gel electrophoresis.